Introduction (TO CANCER)
Before my discovery of the cause of
cancer and other diseases, I had sought to find such
evidence with standard Research microscopes. I observed
all types of malignant tissue to find some trace of the
cause. I felt that the start of malignancy would be
originated by some type of micro-organism.
It became
obvious that in order to find the cause, better means of
observation had to be developed. Thus five microscopes
were designed and built in the laboratory with a range
of 5,000 to 60,000 X. Working in magnifications of
17,000 X and higher revealed new cells and
micro-organisms requiring much skill and patience to
focus and photograph.
After the
isolation of the filtered virus and other pathogenic
organisms, the idea was conceived, that it would be
possible to create an electronic frequency that was in
the correct coordination or resonance of the chemical
constituents of a given organism or virus, and to
devitalize with said frequency, the organism or virus in
question.
The
initial frequency instrument of this nature was first
used and developed in the laboratory in 1920. Due to the
great advancement in the field of electronics, these
frequency instruments have steadily improved to the
present day.
The
isolation of cancer virus and other micro-organisms was
an accomplishment with which I felt a great deal of
pride. Finally in 1931, I discovered the transformation
of cancer virus and the successful treatment for cancer
and other diseases by actual observation of the
universal microscope while applying the frequency
instrument. Thus, this data is presented for evaluation.
With the frequency instrument, no tissue is destroyed,
no pain is felt, no noise is audible, and no sensation
is noticed. A tube lights up and 3 minutes later the
treatment is completed. The virus or bacteria is
destroyed and the body then recovers itself naturally
from the toxic effect of the virus and or bacteria.
Several disease forms may be treated simultaneously.
GENERAL DISCUSSION OF VIRUS OBSERVATION
The major
portion of the cancer tests of the tumors used in the
initial tests were procured from the Paradise Valley
Sanatarium in National City, California. The pathology
of these tumors was checked through their laboratory as
malignant.
The prime
reason that viruses have never been observed in their
true form of their association with a disease is because
the best standard research microscopes will not show
them; first, on account of the lack of great enough
magnification and second, owing to the minuteness of
these particles, it is impossible to stain them with any
known method or technique using acid or aniline dye
stains; hence a substitute stain was found. The viruses
were stained with a frequency of light that coordinates
with the chemical constituents of the particle or
micro-organism under observation.
The
variation of the light frequency is accomplished by use
of a variable monochromatic beam of light that is tuned
to coordinate with the chemical constituents of
particle, virus, or micro-organism is observed by use of
the core beams from the patented Rife Microscope Lamps,
which provide illumination through a series of rotating
quartz prisms in the universal microscope and thence
through the slide containing the specimens and on to the
eyepiece. Rotation of the light beams in the quartz
prisms controls the increase or decrease of the light
frequency. With complete control of the illuminating
unit, a frequency is created that is in coordination
with the chemical constituents of the virus under
observation and thus it is possible to observe the virus
in its chemical refractive index. The control of the
illumination (in the universal microscope and the other
Rife Research microscopes) is a most important factor in
visualizing the virus of any pathogenic micro-organism.
This cannot be accomplished by any conventional sources
of illumination. This points out why other research
groups have failed to find cancer virus.
We
believe and have proven to our satisfaction that the
so-called virus is in reality the premodal cell of a
micro-organism. We also have proven that it is the
chemical constituents and chemical radicals of the virus
under observation which enacts upon the unbalanced cell
metabolism of the body to produce any disease that may
occur. We have in many instances produced all the
symptoms of the disease chemically without the
inoculation of any virus or bacteria in the tissues of
experimental animals.
We have
classified the entire category of pathogenic bacteria
into 10 individual groups. Any organism within its group
can be readily changed to any other organism within the
ten groups depending upon the media within it is fed and
grown. For example, with a pure culture of bacillus coli,
by altering the media as little as two parts per million
by volume, we can change that organism in 36 hours to a
bacillus typhosis showing every known laboratory test
even to the Widal reaction. Further controlled
alterations of the media will end up with the virus of
poliomyelitis or tuberculosis or cancer as desired, and
then, if you please, alter the media again and change
the micro-organism back to a bacillus coli.
METHODS OF CULTURE AND TECHNIQUES OF ISOLATION OF THE
VIRUS OF CANCER
The
methods and principles that were used in this procedure
were as herein related. An unulcerated breast mass that
was checked for malignancy by their laboratory and
ourselves came to our laboratory from the Paradise
Valley Sanitarium of National Cirty, California. The
experiments of 1931 and 1932 were conducted in our Point
Loma Laboratory, then known as the Rife Research
Laboratory.
Ten
millimeter blocks of this tumor (in 1932) were placed in
"K" media and incubated at 37.5 degrees C with no
results. After many long procedures and attempts to grow
the cancer virus had failed, the discovery of the growth
method of cancer virus was found. A test tube containing
a sample from the unulcerated breast mass was sealed and
placed in an argon gas filled loop with 15 mm vacuum and
activated with 5000 volts. This produced a decided
change of ionized cloudiness in the media. (This media
was of tyrode solution and desiccated slime (sic)
intestine). This test tube was then checked for cancer
virus, but at this point none were visible. Then the
test tube was subjected to a 2-inch water vacuum and
incubated for 24 hours. Upon examination the solution in
the test tube was teeming with cancer virus which were
the most highly motile and the smallest in size of any
of the viruses previously isolated.
These BX
or cancer viruses refracted a purplish red color with
the monochromatic beam.
We have
not thoroughly determined the phenomena that takes place
with this technic of culturization, but we believe that
this method brings the organism from the ultraviolet
band into the visibility of refraction. (This method
does not alter the virulence of the virus in any way).
This virus is bi-polar (and will attract to both the
positive and negative poles), but requiring both the + &
- parts to produce a reaction in the tissues of the
experimental animals. Our method used in this procedure
was as follows:
Albino
rats were generally used. The animal chosen for this
experimental work is carried no less than 12 day through
quarantine. The animal is shaved at the point of
inoculation and placed under a partial anesthesia. The
needle for inoculation is filled with triple sterilized
petroleum jelly and the inoculum and passed no less than
20 mm under the epidermis to the point of inoculation.
In 3 to 4 days almost invariably there is an open lesion
which appears in the thyroid area. This recedes at the
end of that time and the growth of the tumor starts at
the seat of inoculation which is a mammary gland. These
tumors develop very rapidly owing to the metabolic rate
of the albino rat. In many cases these tumors have grown
to weight exceeding that of the animal. Upon surgical
removal of this mass and upon microscopical
examination---a true malignancy is shown. That proved
that the virus was pathological. These experiments were
carried through no less than one hundred times with the
same methods and careful technic with the same end
results. We sincerely believe that this leaves no doubt
as to the fact (that the BX organism initially isolated
from the unulcerated human tumor and recovered from the
tumor produced by that BX virus and that BX virus again
recovered) that BX is the primary cause of cancer. We
have in our own classification called this virus of
cancer--BX. We do not expect any laboratory to be able
to produce BX on account of the technic involved and the
lack of adequate optical equipment. This BX or any other
virus cannot be seen with the conventional microscope
and illuminating systems as we have explained often
before. That these tiny live living entities (known as
BX virus) cannot be stained with any of the conventional
acid or aniline dye stains as they are much smaller in
dimension than the molecular particles of said stain and
can be seen only by a frequency of light which
coordinates with their chemical constituents. All
viruses require their own individual frequency of the
mono-chromatic beam to make them visible to the human
eye.
We have
come to the conclusion that the illuminant in the fields
of high powered microscopy is a more important factor
than the high power in magnification of the microscope
because without this source of illumination these
particles called virus are invisible with any amount of
magnification o we have used Koch's postulates in our
methods of recovery which are that the organism
inoculated into the host must again be recovered in its
true form from the host and thus, as stated before this
has been repeated hundreds of times proving to our own
satisfaction that BX or cancer virus is the cause of
malignancy.
This BX
virus can be readily changed into different forms of its
life cycle by the media upon which it is grown.
THE
PROCESS TO PRODUCE THE CANCER VIRUS PHOTOMICROGRAPH (Copyright 1953)
A pure
culture of cancer virus is taken from a known tumor and
filtered through a 000 Berkefeld (sic) W porcelain
filter under 10 mm vacuum. From this filtrate a sample
is drawn off with a thin glass tube which has previously
been heated, sterilized, and drawn to a fine orifice.
One micro-drop is placed on a quartz slide and covered
with a quartz cover slip. The slide is positioned on the
stage of the universal microscope. The universal
microscope is focused on the cancer virus and a 16 mm or
35 mm camera is mounted to expose the (positive)
negatives. The (positive) negatives are developed and
dried and then placed in a 1000 watt enlarger and
exposed for .9 second to a 3 inch by 4 inch glass slide
negative which is developed in microdal fine grain
developer. From this slide, the photomicrograph copies
are reproduced.
CHEMICAL RELATIVITY TO CARCINOMA Coordinative
Constituents
(A)
Dibenzanthracene as a carcinogenetic agent.
1. Di-derivative
of dis[sic, cis?] meaning separated by or doubling up.
2. Benz -
(Benzene C6 H6) Benzol as a C6 H6 derivative C6 H6 nCH2
3.
Anthracene - C14 H10 = 3C6 H6 - C4 H8 white solid
Hydrocarbon used in preparation of indigo and aliza[rin].
(B)
Napthalene (C10 H8) almost the same as C14 H10 (moth
balls)
Cancer Virus Characteristics
-
Not destroyed by X-Ray, ultra violet or infrared
ray.
-
Thermal death point in 24 hours is 42 deg. C or
107.6 deg. F.
-
Sporogenous.
-
Non liquefying (media).
-
Non chromogenic and non aerobic.
-
-
(Cathode) polarization.
-
Width of ovoid or microorganism is 1/20
micrometer.
-
Length of ovoid micro-organism is 1/15
micrometer.
-
Flagellated and non parasitic.
-
Highly motile and plastic.
-
Highly pathogenic.
-
Seen at 12 3/16 degrees angle of refraction on
universal microscope.
-
Color of chemical refraction: purple red, which
results from the coordinative constituents
reaction upon the degree of light frequency
applied.
TECHNIQUE OF "BX" INOCULATION
Our
method of inoculation of experimental animals with "BX",
the virus of cancer, is as follows:
The
animal is first shaved and sterilized with alcohol and
iodine solution at the point of inoculation and placed
under partial anesthesia. This avoids subjecting the
animal to shock. An extra long, very small needle is
used. The needle is filled with the inoculum and the
needle placed in the syringe. The needle is inserted no
less than 30 mm from the point of inoculation to the
epidermis. The point of inoculation is in most cases by
a mammary gland for the reason that the "BX" involved
was recovered from an unulcerated human breast mass.
In 3 to 4
days a lesion appears in the thyroid area. The cause of
this is unknown, but the lesion recedes and heals over
and a growth starts in the mammary gland of the
experimental animal. These growths have exceeded the
weight of the experimental animal in many cases. The
tumor is surgically removed and the "BX" is again
recovered in all cases.
An
important factor and check is to make at least 10
transplants from the initial isolation of "BX". These
transplants are made at 24 hour intervals in the
original "K" media. It increases the virulence and
speeds up the growth of the tumor. With these
experiments that have been repeated on over 100
experimental animals, we are convinced that this method
definitely proves the virulence and pathology of "BX"
virus.
If there
are any workers interested in following this technic, we
will furnish them with the formula of "K" media and all
of the basic principles involved. However, it is beyond
the scope of the average microscope to visualize these
minute virus.
THE
TREATMENT OF "BX" OR CANCER
The
actual cure of cancer in experimental animals occurs
with the use of our frequency instrument. To attain
these astounding results, a long and tedious process is
started to determine the precise setting of the
frequency instrument that is the mortal oscillatory rate
of this virus. When the setting is found, it is repeated
10 consecutive times after the frequency instrument has
been placed back to the same setting before a specific
frequency is recorded. These results are observed under
the high power of the universal microscope and when the
mortal oscillatory rate is reached, the "BX" forms
appear to "blow up" or disintegrate in the field. The
inoculated animals are then subjected to the same
frequency to determine if the effect is the same on the
"BX" virus in the tissues of the experimental animals as
with the pure culture slides; these successful tests
were conducted over 400 times with experimental animals
before any attempt was made to use this frequency on
human cases. (breaks here with a period. The next page
comes after several pages describing viral
characteristics as compiled by Crane from Rife notes and
other information)
...of
carcinoma.
The first
clinical work on cancer was completed under the
supervision of Dr. Milbank Johnson, M.D. which was set
up under the special medical Research Committee of the
University of Southern California. Sixteen cases were
treated at the clinic for many types of malignancy.
After 3 months, 14 of these so-called hopeless cases
were signed off as clinically cured by the staff of five
medical doctors and Dr. Alvin G. Foord, M.D.,
Pathologist for the group. The treatment consisted of 3
minutes duration using the frequency instrument which
was set on the mortal oscillatory rate for "BX" or
cancer (at 3 day intervals). It was found that the
elapsed time between treatments attains better results
than cases treated daily. This gives the lymphatic
system an opportunity to absorb and cast off a toxic
condition which is produced by the devitalized dead
particles of the "BX" or cancer virus. No rise of body
temperature was perceptible in any of these cases above
normal during or after the frequency instrument
treatment. No special diets were used in any of this
clinical work, but we sincerely believe that a proper
diet compiled for the individual would be of benefit.
THE
DETERMINATION AND DIAGNOSIS OF CANCER
We can
determine in over 90% of the cases of persons having
carcinoma by the examination of a blood smear (with the
technic heretofore explained) in 30 minutes. We have
also found that in many types of epithelioma that the
carcinoma tissue carries no conductivity with a pendulum
galvanometer which enables us to outline and determine
the location of a tumor without the use of X-Ray
photographs. It has also been determined that any case
of malignancy treated with either X-Ray or radium or
other radio-active materials shows decided
radio-activity and harmful tissue effects for many
months after the treatments have been given. Destroyed
tissue or tissue that has been harmed is a natural
parasitic feast. We have also found that tumors treated
with this method respond less readily to the treatment
of our frequency instruments.
RESEARCH ON BACILLUS X (CANCER VIRUS) AND METHODS AND
TECHNIC OF ISOLATION
In 1920
to 1925, some 20,000 pathological tissues were sectioned
and stained in the most precise and careful manner, but
failed to show any unknown bacteria or foreign material
under the highest power of our No. 1 microscope.
Attempts were made to culture blocks of tissue taken in
the most sterile manner from an unulcerated breast mass
of proven (BX) malignancy. These blocks were cut in 5 mm
cubes and placed in test tubes containing "K" media.
This media is made from dehydrated, desiccated pig
intestine and a tyrode solution. "K" media has the
faculty of transforming most organisms into their
transitional state and is used with micro-organisms to
liberate their virus or premodal cells.) The tubes were
incubated at various temperatures from 30 to 40 degrees
with no results. Then one of the experiments showed
results. The test tubes were placed in an Argon gas
filled loop excited by 5,000 volts and again examined
after 24 hours. There was a decided change and a
cloudiness in the culture media, however microscopic
examination showed no organisms were visible. By
chemically analyzing the "K" media, it was concluded
that the electronic bombardment had produced an
ionization in the "K" media. To counteract this
ionization, the test tubes were placed in a 2-inch water
vacuum and incubated at 37.5 degrees C for 24 hours.
Subsequent examination at 20,000 X revealed the "K"
media to be teaming with the smallest of any forms
observed. These forms of the cancer virus were called
"BX" and refract a purplish red in a monochromatic beam
of the microscope.
This
method of ionization and oxidation brought the chemical
refraction of the "BX" out of the ultra-violet and into
the visible band of the spectrum. Owingh to the fact
that these test tube specimens had gone through so many
trials, we again started from scratch and repeated this
method 104 consecutive times with identical results. The
"BX" virus was given a complete breakdown to determine
its chemical constituents and characteristics, which are
previously noted in this report.
By
continued microscopical study and stop motion
photography, it was found that the "BX" virus had many
changes and cycles as so with other micro-organisms. The
virus can be readily changed to other forms or cycles of
themselves by the media upon which they are grown. By
altering the "K" media slightly acid, we no longer have
a "BX" as we have classified this cancer virus, but we
have what we term a "BY". In this stage or form, it is
still a virus, but considerably enlarged from the
initial "BX". Still retaining a purple red refractive
index, but will no longer pass the porosity of the W (?)
porcelain or diatomaceous earth filter. In this stage,
the "BY" requires a much coarser "N" filter.
The next
stage finds this micro-organism, now known as the
monococcoid form in the monocytes of the blood of over
90% of carcinomatous individuals. This form can be
readily seen when properly stained with a combination of
a silver nitrate and gentian violet with the standard
research microscope.
As we
change the media again and this time going from a fluid
to a hard base media (using asparagus or tomato agar),
we no longer have a "BX", or "BY", or monococcoid
micro-organism, but we have a cryptomyces pleomorphia
fungi. Any of these forms can be changed back to "BX"
within a period of 36 hours and will produce in the
experimental animal a typical tumor with all the
pathology of true neoplastic tissue, from which we can
again recover the "BX" micro-organism. This complete
process has been duplicated over 300 times with
identical and positive results.
After one
year, we take this same stock culture of dormant
cryptomyces pleomorphia fungi and plant it back on its
own asparagus base media; there is no longer a
cryptomyces pleomorphia, no longer a monococcoid
organism such as is found in the monocytes of the blood,
there is no longer a "BX" or "BY" form, but there is,
from the initial virus isolated directly from an
unulcerated human breast mass, a BACILLUS COLI, that
will pass any known laboratory methods of analysis.
We are
positive from our careful work and technic, that the
causative agent of malignancy can be definitely
identified as bacillus coli as the basic form.
"BX" is a
bipolar virus, that is, retraction occurs to both
positive and negative poles, but both the positive and
negative forms of this virus are required to produce
tumors in experimental animals. We have never publicly
announced that "BX" is the cause of cancer, but we have
succeeded in producing from its inoculation the tumors
as stated before with all the true characteristics and
pathology of neoplastic tissue from which we have
repeatedly recovered the "BX" virus. Many researchers
have attempted to repeat this technic but have failed
for the prime reason of the lack of an adequate
microscope.
THE
LIFE CYCLE AND TREATMENT OF TUBERCULOSIS
The
purpose of this paper is to describe some of the
principles and methods of the isolation and
culturization of the Bacillus of Tuberculosis and its
treatment. This particular organism is one of the more
complicated of the pathogens and its process of
development. We classify this organism in the Fungoid
Group although it is not considered in the same field of
mycology as it has no spores or skis-spores. This
organism was isolated in pure culture in 1879 by Robert
Koch and remains to this day a masterpiece of patient
work. He succeeded in isolating the bacillus of
tuberculosis in the pure form by devising a method of
plating technique. He was the first to contrive and pour
the agar petri dish plates. By this method of isolating
the colonies, as they would appear on the surface of the
plates, he was able in due time to continually produce a
pure strain of the organisms. These organisms in the
pure state pass from the initial rod form through nine
stages in the fungoid group. Most all observers have
seen the more common forms in the branching and mycelium
stages. These forms were recorded and photographs same
were made by the writer and Dr. A.I. Kendall, M.D. in
Dr. Kendall's laboratory in Northwestern University in
1932 and from these initial forms, we succeeded in
isolating by the alteration of the media, the other
eight branching forms. Before succeeding in the
attainment of the virus form, we considered this virus
form as the premodal cell of the bacillus of
Tuberculosis. For example: If the initial rod form of
the organism is inoculated into the experimental animal,
the lymphatic chain produces in from 10 to 12 weeks all
the symptoms of the disease and by inoculating the virus
or premodal cell form, the same symptoms are noticed in
36 to 48 hours. This was repeated many times by the
writer in 1932 with 100% identical results. Tests point
out that this occurs not only with the bacillus of
Tuberculosis, but in many cases with other organisms; in
this form, they also produce the disease. The Universal
microscope shows that virus, under refractive light to
be a jade green in color, highly refractive, and
non-motile. We find with this organism, the same as with
all pathogenic organisms, that if the parent rod is
motile, the virus of that parent rod is motile. If
non-motile, both are non-motile. there is no other form
of these fungoid growths through the complete life cycle
that was found to produce the disease. So we have often
stated, the so-called incubation period of a
micro-organism is in reality a cycle of reversion. Until
that organism grows to a transitional or premodal state,
it does not produce the disease. We have found that this
virus of the bacillus of tuberculosis is the so-called
poison molecule of Voghn. In experimental work with
anti-toxins and vaccines, Vaghn found, as did Robert
Koch, that they could definitely destroy the rod form of
the organism, but the experimental animals would
invariably die. We feel that the phenomena that they
created with their anti-toxins and vaccines was merely
releasing from the rod form, the virus, which in this
form re-enacts upon the dead body of the rod and
produces toxemia and death to the patient. With our
Frequency Instrument treatment for this disease in
question, the devitalizing frequencies of the rod form
and virus form are used simultaneously and the results
attained have been successful on experimental animals
and on human individuals. There is much that can be
accomplished by the continuances of this research and
experimental work on one of the most complicated of the
pathogenic micro-organisms.
-
Signed by Dr. Royal Rife
